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Exploring the impact of permethrin on brain aging and reproduction: model Nothobranchius furzeri

Arnaud, Justine
(2024)

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Arnaud_21491900_2024.pdf
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Details

Supervisors
Page, Melissa ; Donnay, Isabelle
Faculty
Faculté des sciences
Degree label
Master [120] en biochimie et biologie moléculaire et cellulaire, à finalité approfondie
Abstract
Human populations are experiencing increasing longevity, with average life expectancy steadily rising in recent years. However, this extended lifespan implies the emergence of age-related diseases. The onset of these age-related diseases is also favored by exposure to environmental pollutants known to increase the risk of neurodegenerative diseases, impact the development of reproductive organs, and/or disrupt hormonal communication in the reproductive system. These pollutants play a crucial role in the etiology of various health issues, highlighting the intricate relationship between environmental factors and human healthspan. Nothobranchius furzeri, the African turquoise killifish, has emerged as a novel model for aging research due to its rapid life cycle attributed to its evolution in ephemeral habitats, and exhibiting aging characteristics observed in other vertebrates and humans. In this study, we investigated the effects of early life exposure (0 to 7 days post-hatching) to 100µg/L of the widely used insecticide permethrin, on brain aging and reproductive patterns. Reproductive parameters were analyzed separately for male and female fish. Exposure to permethrin did not significantly affect the survival, growth, or reproductive parameters of males and females, although there was high variation in reproductive indexes between replicates. However, a potential deviation in sex ratio was observed, with fewer males in the permethrin-exposed group compared to the control group (DMSO). Acceleration of aging, assessed by mitochondrial DNA copy number, was not observed in permethrin-exposed fish. Despite limitations such as the inability to conduct comprehensive statistical analyses due to a reduced number of aquariums, the variation between aquariums of the same condition was comparable to or greater than the potential treatment effect, suggesting that any potential impact of permethrin on reproduction is minimal.