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Osteogenesis Imperfecta and Sclerostin Antibody - Tendon to Bone Unit Assessment in the oim/oim Mouse

Chretien, Antoine
(2020)

Files

Chretien_Antoine_78141300_2019-2020.pdf
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Details

Supervisors
Behets Wydemans, Catherine
Faculty
Faculté des sciences de la motricité
Degree label
Master [120] en sciences de la motricité, orientation générale
Abstract
Introduction. Osteogenesis imperfecta (OI) is a genetic disorder of type I collagen. This preliminary study aims at describing tendon to bone unit in OI mice and the effect of antibody neutralizing sclerostin (Scl-Ab). Methods. Histological sections were made through the insertions of triceps brachialis, patellar ligament and triceps suralis of Control OI (OIC) and Wild type (WT), and of OI and WT treated with Scl-Ab for 9 weeks. The sections were stained with Sirius red or toluidine blue. Results. In OIC, epiphyseal bone showed low BV/TV as well as deformations. Tendon had a higher proportion of thick collagen fibers and could present islets of fibrocartilage. The tidemark between unmineralized and calcified fibrocartilage was shorter than in WT. In Scl-Ab treated OI mice, BV/TV increased and the proportion of thin collagen fibers significantly increased in tendon and fibrocartilage. Discussion and conclusion. This histological study highlighted original features of enthesis in OI which could explain tendon ruptures in patients. Scl-Ab treatment appeared to impact positively bone epiphysis but also some other components of enthesis. Higher resolution imaging and mechanical tests are needed to confirm these preliminary data.