IDENTIFICATION DES BACTERIES DU MICROBIOTE RESPIRATOIRE HUMAIN LIEES AUX ANTICORPS IgA : Evaluation technique et application à la rhinosinusite.

Details

Supervisors

Pilette, Charles ; Hox, Valérie

Faculty

Faculté de pharmacie et des sciences biomédicales

Degree label

Master [120] en sciences biomédicales, à finalité approfondie

Abstract

Chronic rhinosinusitis (CRS) is an inflammatory disease of the mucosa of the nose and paranasal sinuses lasting more than 12 weeks, characterized in two forms, namely CRS with polyps (CRSwNP) and CRS without nasal polyps (CRSsNP). Both phenotypes manifest with a spectrum of symptoms, the most differentiated of which are nasal obstruction, rhinorrhea, facial pain/pressure, and loss of smell leading to a significant disease burden. Previous work has shown that dysbiosis could be one of the pathophysiological mechanisms explaining the occurrence of this disease. Since the main antibodies that regulate mucosal immune responses are IgA, a fraction of the commensal microbiota is covered with IgA under homeostatic conditions. In the gut, IgA acts in a context-dependent manner to prevent, promote bacterial colonization and influence bacterial gene expression, thus allowing a regulated control of the microbiota. This study allowed us to develop the technique to identify bacteria in the airway epithelial mucosa microbiota bound by IgA antibodies in chronic inflammatory airway diseases such as CRS.