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Study of the Mn2+-mediated transcriptional regulation of GDT1

Dubois, Marion
(2024)

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Dubois_26131800_2024.pdf
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Details

Supervisors
Morsomme, Pierre ; Lejeune, Quentin
Faculty
Faculté des sciences
Degree label
Master [120] en biochimie et biologie moléculaire et cellulaire, à finalité spécialisée: biotechnologie
Abstract
The Gdt1 family is composed of transporter proteins. This family includes the human ortholog, TMEM165, localized at the Golgi apparatus membrane. It was highlighted in 2012 that deficiencies in this protein can cause Congenital disorders of glycosylation (CDG). This disease is characterized by a defect in glycosylation processes and can lead to multisystemic symptoms. The yeast ortholog, Gdt1p is also localized at the Golgi apparatus membrane. Gdt1p and TMEM165 are both responsible for the transport of Mn2+, Ca2+, and H+ across de Golgi apparatus membrane. These two transporters were shown to be degraded in presence of high manganese concentrations in the extracellular medium. Moreover, Gdt1p is not only affected at the protein level as it was shown that the GDT1 expression is also downregulated in these conditions. In eukaryotes, such Mn2+-induced transcriptional regulation has not been identified to date. During this project, the regulation of the mechanism of expression was studied. More precisely, the involvement of the promoter in this mechanism was assessed. The promoter activity was studied via the Dual luciferase reporter system, adapted for utilization in yeast. Furthermore, this study has contributed to the identification of transcription factors (TF) involved in this transcriptional regulation process via analysis of GDT1 expression in TFs knockout mutants.