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Species boundaries and adaptive potential in Ellobiids snails

(2025)

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Abstract
The study explores the cryptic species complex of Melampus bidentatus through karyotyping, with a focus on resolving the species boundaries and understanding the evolutionary processes involved. Karyotype analysis was initially attempted using colchicine treatment to induce cell division, but no conclusive results were obtained, possibly due to issues with tissue digestion, cell resistance to treatment, or slide preparation. Additionally, the study investigates salinity responses through RNA-seq analyses. Snails, Myosotella sp., were exposed to varying salinities, and differential gene expression was analyzed across three conditions (5, 20, and 35 ppt). However the data could not be ready in time, thus data analyses was made on Melampus jaumei, species part from de cryptic complex, exposed to various salinity conditions (5, 20 and 35 ppt). This work contributes to the understanding of genetic mechanisms underlying salinity tolerance in littoral gastropods to contribute to the understanding of geographical partitioning and many future investigations are possible.