ATTENTION/WARNING - NE PAS DÉPOSER ICI/DO NOT SUBMIT HERE

Ceci est la version de TEST de DIAL.mem. Veuillez ne pas soumettre votre mémoire sur ce site mais bien à l'URL suivante: 'https://thesis.dial.uclouvain.be'.
This is the TEST version of DIAL.mem. Please use the following URL to submit your master thesis: 'https://thesis.dial.uclouvain.be'.
 

Development of an enzyme immobilization system combining LbL and Avidin-biotin interaction

De Meeûs d'Argenteuil, Juliette
(2023)

Files

deMeeus_20561800_2023.pdf
  • Open access
  • Adobe PDF
  • 16.44 MB
Download

Details

Supervisors
Dupont-Gillain, Christine C. ; Demoustier-Champagne, Sophie
Faculty
Faculté des bioingénieurs
Degree label
Master [120] : bioingénieur en chimie et bioindustries, à finalité spécialisée
Abstract
This work presents a versatile method for enzyme immobilization utilizing the avidin-biotin specific interaction to immobilize a biotinylated-enzyme inside a Layer-by-Layer (LbL) film of Avidin. Nanoporous polycarbonate membranes serve as substrate to benefit from the large specific area developed by such materials. First, the assembly of different systems on a flat surface was monitored using Quartz Crystal Microbalance measurements. Subsequently, activity and stability comparisons were conducted between different system architectures to identify the most promising approaches. Based on these evaluations, two constructions with superior activity and stability were selected for further assessment through LbL film assembly onto membranes. In a second phase, the efficiency of the two optimal LbL constructions on nanoporous membranes was assessed and compared. A comprehensive methodology was developed to obtain multiple pieces of information, including the theoretical amount of immobilized enzyme and protein, the effective activity of the assembled membrane in static mode, and the extent of enzyme leaching from the membrane. The results demonstrate the successful assembly of enzyme-immobilized systems on both flat surfaces and nanoporous membranes using the avidin-biotin assembly method. The comparative analysis indicates improved activity and stability compared to traditional LbL systems. Overall, this study highlights the potential of the avidin-biotin assembly technique for enzyme immobilization on nanoporous membranes. The findings contribute to the development of innovative approaches in enzyme immobilization technology, with potential applications in various fields such as biocatalysis, biosensing, and bioseparations.

Citations

  • APA

De Meeûs d’Argenteuil, J. (2023). Development of an enzyme immobilization system combining LbL and Avidin-biotin interaction.