Isolation and characterization of bacteriophages infecting Xanthomonads

Details

Supervisors

Gillis, Annika ; Bragard, Claude

Faculty

Faculté des bioingénieurs

Degree label

Master [120] : bioingénieur en sciences agronomiques, à finalité spécialisée

Abstract

This research focuses on isolating and characterizing Xanthomonas-targeting bacteriophages to evaluate their efficacy against bacterial infections in wheat and cassava crops. Diverse Xanthomonas-specific phages are identified and characterized from environmental samples, offering insights into their host specificity and therapeutic potential. Standard laboratory procedures are employed to culture Xanthomonas bacteria, including controlled cultivation of reference strains. Phage isolation methods are used to extract phages from collected environmental samples. Drop tests are conducted to evaluate phage infectivity on agar plates, followed by the isolation and characterization of individual plaques to assess phage diversity and stability. The findings significantly contribute to the field by demonstrating the broad host range and therapeutic potential of Xanthomonas-specific phages. The study also explores the thermostability and pH stability of selected phages, providing practical insights for their potential agricultural applications. These results establish a solid foundation for future research in Xanthomonas phage therapy and biocontrol strategies. By recognizing limitations and challenges, this research offers valuable insights into the scope of the study and identifies opportunities for future growth. Further research should investigate the genetic and molecular factors influencing bacterial susceptibility to phage infection, optimize phage therapy strategies, and explore the ecological implications of phage-based interventions. Moreover, advancements in high-resolution imaging and other innovative approaches hold promising prospects for enhancing our understanding of Xanthomonas phages and developing tailored phage therapies. Overall, this master's thesis sheds light on the interactions between Xanthomonas bacteria and phages, providing important insights into phage susceptibility, host range determination, and their applications in biocontrol strategies. The research emphasizes the significance of collaboration, responsible phage use, and innovative approaches to address antibiotic resistance and optimize the effectiveness of phage-based interventions. It represents a significant advancement in the field of phage therapy and biocontrol strategies, contributing to the effective management of Xanthomonas and related bacterial pathogens in agriculture.