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Lakhdar_84341700_2014.pdf
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- This study explores innovative approaches for the fabrication of thin biofilms with controlled architecture, emphasizing the potential of Gas Cluster Ion Beam (GCIB) technology in (bio)materials processing. The research investigates three primary concerns within the GCIB-assisted deposition process particularly for enzymes. The first concern addresses the transfer of lysozyme via GCIB, examining the influence of argon cluster size on the transfer efficiency and subsequent bioactivity. Findings indicate a proportional relationship between the number of active lysozyme transferred and the cluster size, with the highest efficiency observed for Ar5000+. However, significant differences between activity and BCA tests highlight limitations in quantifying fragmented proteins, prompting the recommendation for alternative quantification methods. The second focus involves the transfer of GOx (monomeric structure of ≈65 kDa) via GCIB, indicating the adequacy of the method for larger proteins. This is the first study demonstrating the succesful desorption of GOx from a silicon surface using a 10 keV Ar5000+ primary beam and the collection of active enzyme on a PE collector. Further investigations, such as native PAGE analysis, are recommended to validate the preservation of the protein structure. The final aspect explores the construction of organic multilayer systems via GCIB, highlighting the successful transfer of GOx and β-D-glucose onto a silicon wafer. Depth profiling analysis confirms the successful transfers, providing insights into the molecular characteristics of the multilayer. Bioassay results support the retention of enzymatic activity after transfer, showcasing the feasibility of building functional multilayer systems. Stability assessments over time demonstrate the enduring nature of organic multilayers, opening avenues for future studies and applications in biotechnology.