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AkBA, a natural antioxidant, increases protein synthesis in human myotubes

De Groote, Estelle
(2017)

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Details

Supervisors
Deldicque, Louise ; Rodriguez, Julie
Faculty
Faculté des sciences de la motricité
Degree label
Master [120] en sciences de la motricité, orientation éducation physique
Abstract
Background: A significant loss in skeletal muscle mass is associated with a poor quality of life and an increase in mortality rate. Consequently, therapeutic strategies need to be developed to assist in reversing or limiting the loss of muscle mass. Reducing oxidative stress could be one strategy as some studies evidenced a link between this specific stress and muscle atrophy. Boswellia serrata, a natural compound that has anti-inflammatory and antioxidant properties, could be a good candidate to fight the loss in muscle mass associated with oxidative stress. Purpose: We aimed to highlight the potential efficacy of AkBA, a boswellic acid found in Boswellia serrata extract, for the treatment of muscle mass loss associated with oxidative stress in human myotubes. Methods: Human myotubes were incubated with AkBA for 24h, hydrogen peroxide (H2O2) for 4h or16h, both treatments (AkBA + H2O2) or DMSO for 24h. Specific markers of protein synthesis, autophagy and oxidative stress were measured by Western blot (proteins) and qPCR (mRNA). Results: Our study reveal that AkBA induced an increase in protein synthesis in human myotubes after 24h of incubation (P < 0.01). The mechanisms of action of AkBA on protein synthesis were independent of mTOR and AkBA had no effect on autophagy. Treatment with AkBA attenuated the decrease in catalase and MnSOD content induced by H2O2 and decreased Nox4, Nox2 and PGC1α gene expression compared with H2O2 conditions. Conclusion: We found a new compound able to increase protein synthesis in human myotubes. In addition, this study highlights the potential benefits of AkBA as an antioxidant.

Citations

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De Groote, E. (2017). AkBA, a natural antioxidant, increases protein synthesis in human myotubes. https://dial-mem.test.bib.ucl.ac.be/handle/2078.2/11620